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CAS No. | 126208-12-6 | Cat. No. | BCP48281 |
Name | Snarf-1 | ||
Synonyms | Carboxy-SNARF 1 5/6-mixture; 5-(6)-Carboxy RhodFluor; | ||
SMILES | [H-].CN(C)C1=CC2=C(C=C1)C(=C3C=CC4=CC(=O)C=CC4=C3O2)C5=CC=CC=C5C(=O)O | ||
Chemical Name | |||
Formula | C27H19NO6 | M. Wt | 453.45 |
Purity | 98% | Storage | Store at 4-8°C |
Description | The cell-permeant pH indicator, carboxy SNARF-1, acetoxymethyl ester, acetate has a pKa of ~7.5 after de-esterification, thus is useful for measuring pH changes between pH 7 and pH 8. Carboxy-SNARF-1 exhibits a significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectively. This pH dependence allows the ratio of the fluorescence intensities from the dye at two emission wavelengths – typically 580 nm and 640 nm – to be used for quantitative determinations of pH. |
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